Spiral plater for bacterial enumeration


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Spiral Plater
Accelerated Bacterial Colony Enumeration

Features of the Spiral Plater for bacterial enumeration

Rapid Bacterial enumeration without intermediate dilutions.
Economical - save on Petri dishes, agar, pipettes.
Automatic sample taking, disinfecting and rinsing.
250 ul of sample drawn in one cycle for plating. successive dishes: 5 x 50 ul.
Multiple replicate plates prepared consecutively without take up of additional sample.
Operating modes: automatic, semi automatic or manual.
Plating modes: standard exponential, slow exponential, proportional, uniform and lawn.
Many safety features.
Continuous supply of disinfecting liquids
Bacterial enumeration up to 4,000,000 cfu/ml on 150 mm Petri dishes

Introduction to Interscience Spiral Bacterial Plater
The Spiral DSPLUS is a microprocessor controlled dispenser that deposits a liquid sample in a spiral pattern onto the surface of a rotating agar plate. The dispensing stylus deposits decreasing amounts of sample as it moves from the center of the plate outwards. Because of this unique plating action, microbial suspensions (~ 400 to 400,000 cfu/ml on 100 mm plates ) that may normally require several serial dilutions prior to plating can be plated directly The spiral method allows rapid bacterial counting while avoiding some or all intermediate dilutions. A logarithmically decreasing volume of sample is dispensed on the surface of a rotating Petri dish in an Archimedes spiral. The volume is calibrated and is known at every point of the Petri dish. The bacterial concentration is determined by dividing the number of colonies found by the volume dispensed in the same sector of the dish.

Five Deposition Modes
The Spiral Plater deposits sample is five different deposition modes on both 100 mm and 150 mm plates. Operation can be automatic, manual or semi automated. Automatic mode features hands free cleaning and sample intake, while manual modes can be used to intake viscous or particulate samples or samples from nonstandard containers.
Spiral plates can usually be counted after only 18 to 24 hours incubation (depending on types of microbes ). Cfu/ml of sample is determined by manually counting colonies in two opposite sectors of a specialized counting grid, for which the deposited volumes are known. Or by automated counting.

	Standard Exponential Mode Causes exponentially decreasing amounts of sample to be deposited for bacterial enumeration by spiral plating method and for single colony isolation. Also used to deposit anti-microbial agents in the Spiral Gradient Endpoint test and to produce a gradient of mutagenic substances in the Spiral Salmonella Assay.
	Slow Exponential Mode Used when plating organic compounds such as ethanol, or when using wet plates - prevents spirals from being splashed by centrifugal force.
	Proportional Mode Deposits decreasing amounts of sample proportional to the radial distance from the center or the plate. The range of concentrations is therefore much smaller than for exponential mode. Useful for amplifying the response of bacterial populations to anti-microbial agents or mutagens over the narrow range of their gradient.
	Uniform Mode Causes the sample to be deposited at a constant rate over the entire spiral path. Used for plating the Salmonella in the Spiral Salmonella Mutagenicity Assay
	Lawn Mode Produces uniform contiguous growth on the entire plate by depositing the inoculum at a constant rate as in the uniform mode but at half the spacing between the spirals. Useful for test requiring precise and even distribution of substances or microbes across a plate such as disk diffusion tests and studying viral plaques or enzyme production

Specifications of the DS Plus Spiral Plater

Plate Diameters	90 - 150 mm
Volume dispensed	16 - 55 ul
Counting range cfu/ml	200 to 400,000 for 90 mm Petri dishes up to 4,000,000 for 150 mm dishes
Dispensing time	8 seconds
Vacuum required	50 - 70 cm Hg
Weight	22 kg
Dimensions	600 x 280 x 400 mm
Power	120/250 VAC

Spiral Plater applications
Food Hygiene control, Water and milk testing, medical analysis, cosmetics pharmaceuticals

Accessories
Beakers for Spiral Plater Samples Sterile polypropylene sample cups 5 ml in sterile packs of ~ 67 beakers ( US quarter for scale )

Ordering Information Interscience Spiral Plater

Model	Description	Part No	Price USD
DSPLUS	Spiral plater	DSPLUS	$19950.00
DS Vac	Bench Top Vacuum	DSVAC	$2100.00
Godet	Sterile Beakers 1000 pack ~ 67/sterile tube.	DB-50	$145.00
PetriPile	Rack for petri dishes 55, 65 or 90 mm	PP90	$75.00

Topac Inc 231 CJC Highway, Cohasset MA 02025 USA
Tel: 781 740 8778 Fax: 781 740 8779 e-mail: sales@topac.com