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UV-VIS Spectrophotometer

Features of the UV VIS Spectrophotometer
  • 190 - 900 nm range
  • 2 or 5 nm Bandwidth
  • Jobin Yvon Monochromator
  • 10 - 100 nm long path length single cell holder
  • Compact - small footprint - large sample compartment
  • LCD graphic displays up to 8 spectra simultaneously
  • Built in applications software
  • Aspiration ( sipper ) systems with temperature control option
  • Automatic cell changer
 
spectrophotometer

Introduction to Spectrophotometers
The Anthelie series of UV - Visible spectrophotometers from Secomam are high quality feature rich instruments suitable for industrial and academic research laboratories in the fields of biology, chemistry, pharmacy, environmental and education. The range of instruments offer outstanding photometric characteristics, integrated software with built in applications and a graphics display. The instrument is available in two version 'Light' (1 nm resolution 2 line display) and 'Advanced' (0.1 nm resolution graphic display ). Each version is available with either 5nm or 2 nm bandwidth. See the table below for a detailed comparison of features.

High Quality Optics
All units include a Jobin Yvon monochromator with a 1200 l/mm holographic grating

Ergonomic Design
The unique design of the instrument case provides a spacious sample compartment. In addition the the whole outer case of the instrument swings upwards allowing instant access to the interior of the unit for the installation of accessories and changing the lamp.

Sample Holders and Accessories
auto samplerThe standard sample holder is a single cell holder for square or rectangular cells with an optical path from 10 nm to 100 mm in length. Even weakly colored solutions such as those found in sugar refining can be measured with this system.. Automated measurements can also be performed with the automatic sampler accessory which holds up to nine 10 mm square cells.

Graphic Display
graphic displayThe advanced models are fitted with an LCD graphical display where you can view up to 8 spectra simultaneously and use zoom, cursor and peak search functions. The display can also be used for navigation through the software menus and to set up plots for dynamic experiments such as absorbance vs time.

New Applications
DNA Concentration
Anthelie Light performs simple calculations such as absorbance ratio (Abs260nm / Abs280nm) for DNA purity or absorbance differences (Abs1
ûAbs2) for DNA concentration. 

     You can create your own custom mathematical formula with a combination of up to 3 wavelengths; without internal reference (Abs260nm / Abs280nm) or with internal reference (Abs260nm ûAbs320nm) / (Abs280nm ûAbs320nm). Furthermore. any custom mathematical formula can be programmed and saved under a specific user name. 
     
Anthelie Advanced  simultaneously displays each individual absorbance along with the calculated result.

The absorbance / transmittance measurements made in this type of spectrophotometer are based on Beer's Law - you can learn more about Beer's law here
   
Specifications

Aurius Specification

Monochromator Littrow using 1200 L/mm holographic grating
Optical Bandwidth   4nm
Wavelength Range   190-1000nm; 325-1000nm visible versions
Wavelength Accuracy   Better than ±1nm
Wavelength Reproducibility   0.1nm
Self Test and Calibration   Automatic at switch on
Straylight   Typically less than 0.01% at 220nm and 340nm
Display Screen - LCD   High resolution backlit with contrast control
Display Scrolling   Provides 6 screen widths for viewing plots etc.
Autozero   Automatic by press button
Photometric Ranges   Digital display of -0.3-3A, 0-200%T, 0-9999C
Photometric Accuracy   ±0.005A or 1% whichever is greater
Photometric Noise   Less than ±0.0002A (500nm)
Stability   Better than 0.001A/hour at constant temperature
Multi-Wavelength Analysis   2 and 3 wavelength methods.
Assay Program   Enter multi-wavelength formula for automatic computation and formatting of any assay
Curve Fitting and Editing   Linear, quadratic or cubic; up to 30 standards
Cell and Wavelength Programming   Up to 8 cells and 10 wavelengths combined
Method Storage   Up to 30 methods stored in protected memory
Absorbance Ratioing   260/280nm or two selected wavelengths
Peak Seek   Automatic search programme, baseline corrected
Reaction Kinetics   Tabulation of results, reaction plots, reprocessing
Batch Sampling   100 samples, internal control, screen display
Real Time Clock   Timed and dated reports
Computer/printer interfaces   Bi-directional serial RS232 and parallel ports
Sample Temperature   Displayed and set from keyboard
Size   480 x 340 x 205mm
Weight   16.8Kg Visible; 18.6Kg UV; + 0.9Kg with printer
Power Requirements   110-250V, 50/60Hz, 170W/120W UV/Visible versions
     

SuperAurius Additional Specification

Optical Bandwidth   1.8nm
Wavelength Scale Expansion   Selectable by keyboard entry 1-100nm/cm
Scan Speed   Selectable by keyboard entry 1-4000nm/m
Photometric Plotting Ranges   Keyboard entry of limits; or automatic optimum
Baseline Flatness   Better than ±0.002A (300nm/min) most of range
Overlayed Spectra   Spectra and derivatives with or without offset
Spectral Reprocessing   Spectra manipulated and replotted over any range
Spectral Storage   Up to 100 spectra may be stored security code protected
Derivative Spectra   1st and 2nd derivatives
Time Course Plotting   Plots may be reprocessed and stored
 
Methods
 
Function Basic Instrument Advanced Instrument
Absorbance / Transmittance Real time measurement Real time measurement
Concentration Linear regression
1 to 8 standards
Factors up to 9999
Sample blank
Linear regression
1 to 8 standards
Factors up to 9999
Sample blank
Sample and reagent blank
Linear or Log axis
Calibration curve displayed
Point restitution suppression
15 selectable units
Kinetics Initial delay ( lag time )
0 - 240 secs
1 - 25 segments
segment time 0 - 240 secs
Factor or standard calibration
0 - 60 secs
total time up to 8 hrs
Activity factor up to 9999
Slope and activity display
Slope recalc of linear portion
Zoom on specific curve parts
15 selectable units

Overlay up to 8 curves
Time course absorbance Total time up to 8 hours
Multi-wavelengths Up to 2 wavelengths
individual wavelengths absorbencies
Ratio and difference calculation
Up to 3 wavelengths
individual wavelengths absorbencies
Free calculation formula
DNA ratio 260 nm/280 nm
or (260-320) / (280-320)nm
Spectral Scanning Basic scanning fixed speed 1200 nm/min
Graphic output to printer
Advanced scanning
20, 500, 1200, 2400 nm/min
Abs or %T acquisition mode
zoom, cursor, Abs ~ %T conversion
Auto peak detection
Overlay up to 8 spectra
Memory 50 Methods 
Alphanumeric naming
30 Methods, 8 curves
Alphanumeric naming
Display 2 line backlit LCD 320 x 240 pixel graphic LCD with contrast control. (90 mm x 120 mm )


Ordering Information
Model Description Part No Price USD
Anthelie Light 5 Basic unit 5 nm bandwidth T70STO376 $4600.00
Anthelie Light 2 Basic unit 2 nm bandwidth T70STO422 $5100.00
Anthelie Advanced 5 Advanced unit 5 nm bandwidth T70STO375 $5250.00
Anthelie Advanced 2 Advanced unit 2 nm bandwidth T70STO410 $5850.00
 
Accessories
Accessories Description Part No Price USD
Sipper ( aspiration ) system visible 80 ul glass cell includes 80 ul flow through glass cell, peristaltic pump and tubing kit. T70M10314 $1100.00
Sipper ( aspiration ) system UV Visible 80 ul quartz cell includes 80 ul flow through quartz cell, peristaltic pump and tubing kit T70M10315 $1350.00
Peltier Temperature Control for sipper 80 ul quartz cell includes 80 ul flow through quartz cell, peristaltic pump and tubing kit, thermostat 10 cm cell holder. Manual temperature control switch T70M10303 $2250.00
9 position multi-cell holder holds up to 9 10 mm square cuvettes. requires auto cell changer below T70M10294 $375.00
Automatic multi cell changer Automatic Cell Changer for multi cell holder. ( only for 'Advanced' model ) T70ST0397 $595.00l


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