UVIband & UVImap : Common functions
- Image to be analyzed may be chosen from "montage"
reduced images.
- Image may be any one of ten file types (TIFF, BMP, GIFF,
etc.),
- Image manipulations include mirror, zoom, rotate, invert,
stretch contrast, cut and paste.
- Semi-automatic or manual lane definition with full editing
capability.
- Two methods for correction for local and global gel distortions
("smiles", "frowns", etc).
- Molecular weight/fragment size and pI calculation from standards
plus RF calculation
- Band quantification with fully automatic subtraction of
irregular background form across the entire gel.
- Calculation of relative band volumes using local or global
references.
- Matrix/dendogram calculation and band matching facilities allow
reliable comparison of band patterns within a gel
- Lane profile overlay facility and bar chart or graph
representation of volume data.
- Ability to save analysis results files with GLP information.
Data files exportable to database programs
- Results can be automatically printed out in single sheet format
for notebook.
UVIband : Additional features
- Several modes of background subtraction (including automatic)
for quantification.
- Definition of band or spot edges by accurate contour
recognition.
- Accurate band volume calibration by construction of calibration
curve.
- On-line spreadsheet for automatic data manipulations.
- Automatic quantitation facility for dot/slot blots or microtitre
plate experiments.
UVImap : Additional Features
- Comprehensive database facility with password protection for
storage of molecular weight/fragment size or RF data.
- Ten levels of directories in database, up to 999 directories per
level.
- Facility for Comparison of positional data from lanes of
different gels using matrix and dendogram analysis.
- Ability to construct a reference "master lane" for
comparison with stored or current lanes.
- Multiprobe analysis possible by production and combination of
"binary" (presence/absence) files.
- Search facility to identify all lanes in database directory
which meet similarity criteria compared to specified lane
UVIspot : The inexpensive 2D alternative
- Comprehensive 2D analysis at a 1D price
- Automatic detection of ‘matched’ spots
- Gel comparison by spot movement, volume variations and presence
or absence
- comparison of up to 256 gels in one experiment
- Up to 9 parameter spot characterization
- Searchable database for easy archiving and comparison
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