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Harvard/Amika ElectroPrep
Rapid purification of proteins nucleic acids and all kinds of biomolecules
Electroelution apparatus Electro-elution  Features
  • Electro-elution from gels and solutions

  • Electro-dialysis (with an average buffer exchange time of 5 to 10 minutes)

  • On-line electro-dialysis

  • Electro-concentration
  • Selective electro-filtration
  • Size fractionation

 
The ElectroPrep system is an extremely versatile sample prep technology developed by Harvard/AmiKa scientists. This patented, electrophoresis-based system is ideal for the rapid purification of proteins, nucleic acids, carbohydrates and other biomolecules. With a run-time of 5 to 10 minutes. ElectroPrep provides speed and convenience, even at the very low currents (5 to 10mA) used with this system. The sample and elution chamber are both made of PTFE, a completely, inert material especially suited for high sample recovery. Membranes of differenct MWCO (molecular weight cut off), from 100 to 300,000 Daltons, can be used for selective elution, filtration, dialysis, fractionation and concentration. See below for a full list of membranes available.

Multiple link chambers can be joined together with membranes of different MWCOs placed between them for highly selective electro-filtration and separation
Electroelution  More Features
  • Primer removal
  • Salt removal
  • Detergent removal
  • Dye-Terminator removal
  • Faster dialysis times due to movement of charged molecules in the electric field
  • Re-usable
  • Available for most sample sizes large or small
  • Membranes available with MWCO's to suit almost any application
  • Easy to use
  • leak proof
  • PTFE parts are autoclaveable
  • low protein binding
  • High sample recovery
  • Made of PTFE - totally inert

pdf data sheet
 
 
Ordering Information
ElectroPrep Tank EP-74-101  
Power supply MPSU-200/100   
Union, 25-200 ul to 25-200 ul EP-74-0100   
Union, 250-1500 ul to 250-1500 ul EP-74-1105   
Union, 25-200 ul to 250-1500 ul EP-74-0102   
  1/pack 5 /pack
BioDialyser Turbo, 50ul EP-74-0408 EP-74-0400
BioDialyser Turbo, 100ul EP-74-0409 EP-74-0401
BioDialyser Turbo, 200ul EP-74-0410 EP-74-0402 
BioDialyser Turbo, 500ul EP-74-0411 EP-74-0403
BioDialyser Turbo, 1000 ul EP-74-0412 EP-74-0404 
 BioDialyser Turbo, 1500 ul EP-74-0413 EP-74-0405
Link Chamber, 25 ul EP-74-1619  EP-74-1620
Link Chamber, 50 ul EP-74-1611 EP-74-1615
Link Chamber, 100 ul EP-74-1612 EP-74-1616
Link Chamber, 250 ul EP-74-1613 EP-74-1617
Link Chamber, 500 ul EP-74-1614 EP-74-1618
 
 

Membranes
A large variety of membranes are available for use with the ElectroPrep unit. The following tables are designed to assist you in choosing the appropriate membranes for your needs.

Cellulose Acetate
These membranes are low protein binding and have a sharp MWCO range. The membranes are pre-cut, pre-washed, and supplied in 0.05% sodium azide solution. They are ready to use without any further treatment. Glycerol, sulphur, and heavy metals are not present in these membranes. The cellulose acetate membranes are intended only for aqueous solutions, and the presence of an organic solvent is not recommended.

Regenerated Cellulose
These membranes are more stable in organic solvents, but the MWCO is not as precise as for cellulose acetate membranes and are not offered over as wide a range of MWCO. The membranes are pre-cut, pre-washed, and supplied in 0.05% sodium azide solution. They are ready to use without any further treatment. Glycerol, sulphur, and heavy metals are not present in these membranes.

Polycarbonate
These membranes are more stable in organic solvents. They are available in three highly controlled pore sizes for a well defined MWCO range.
 
Membranes for Dialysers with Size range of 10 ul to 200 ul
Membrane Cellulose Acetate Regenerated Cellulose Polycarbonate  
MWCO (kDa) pk/24 pk/96 Pk/24  Pk/96 Pk/24 Pk/96
0.1  EP 74-2410  EP 74-2400        
0.5 EP 74-2411  EP 74-2401        
1  EP-74-2412  EP74-2402 EP 74-2706 EP 74-2700    
2  EP 74-2413  EP 74-2403 EP 74-2707 EP 74-2701     
5  EP 74-2414  EP 74-2404 EP 74-2708  EP 74-2702     
10  EP 74-2415  EP 74-2405 EP 74-2709 EP 74-2703     
25  EP 74-2416  EP 74-2406 EP 74-2710  EP 74-2704     
50  EP 74-2417  EP 74-2407 EP 74-2711 EP 74-2705    
100  EP 74-2418  EP 74-2408        
300  EP 74-2419  EP 74-2409        
Membrane Pore Sizes
0.01 um         EP 74-3103 EP 74-3100
0.05 um          EP 74-3104 EP 74-3101 
0.60 um         EP 74-3105 EP 74-3102

Membranes for Dialysers with Size range of 250 ul to 1500 ul
Membrane Cellulose Acetate Regenerated Cellulose Polycarbonate  
MWCO (kDa) pk/24 pk/96 Pk/24  Pk/96 Pk/24 Pk/96
0.1  EP 74-2510  EP 74-2400        
0.5 EP 74-2512  EP 74-2401        
1  EP-74-2513  EP 74-2402 EP 74-2806 EP 74-2800    
2  EP 74-2514  EP 74-2403 EP 74-2807 EP 74-2801     
5  EP 74-2515  EP 74-2404 EP 74-2808  EP 74-2802     
10  EP 74-2516  EP 74-2405 EP 74-2809 EP 74-2803     
25  EP 74-2517  EP 74-2406 EP 74-2810  EP 74-2804     
50  EP 74-2518  EP 74-2407 EP 74-2811 EP 74-2805    
100 EP 74-2519  EP 74-2408        
300  EP 74-2520 EP 74-2409        
Membrane Pore Sizes
0.01 um         EP 74-3203 EP 74-3200
0.05 um         EP 74-3204 EP 74-3201 
0.60 um         EP 74-3205 EP 74-3202

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