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Mini Fluorometer

The Turner TBS-380 Mini Fluorometer is an economical, sensitive fluorometer designed for quick, easy, and accurate fluorescence measurements. The new Minicell Adaptor enables you to measure samples at low concentrations with an assay volume as low as 50 µL.

Hand held oxygen monitor

Features of the Minifluor

· Dual-channel (UV & BLUE)
· Serial interface
· Two reading modes (Discrete & Continuous)
· Internal Data Logging Software
· Solid Standard
· Minicell Adaptor (optional)
· Single-point calibration

Primary Fluorescence Applications
Nucleic acid quantitation: *PicoGreen^®, RiboGreen^®, and Hoechst 33258 dye.
Protein quantitation: *NanoOrange^®Enzyme Studies: 4-Methylumbelliferone^{*Trademarks : PicoGreen, RiboGreen, and NanoOrange are registered
trademarks of Molecular Probes, Inc.}

Sensitive Fluorometer
The TBS-380 Mini-Fluorometer provides high sensitivity for fluorescence measurements. The new Minicell Adaptor with two focusing lenses allows you to measure samples in low assay volumes without losing sensitivity. When used with the Minicell Adaptor, the TBS-380 Mini Fluorometer can detect 50 pg of dsDNA in 50 µL assay volume using PicoGreen^® reagent.

TBS-380 Mini-Fluorometer Minicell Adaptor Kit

Measure sample volumes as low as 50 µL without losing sensitivity.
Adaptor fits into the TBS-380 Mini-Fluorometer sample compartment.
Easy to use.

Overview
For applications where sample volume is a concern, the Minicell Adaptor with two focusing lenses allows you to measure sample in an assay volumes as low as 50 µL without losing sensitivity (e.g. 1 ng/mL dsDNA using PicoGreen, 50 pg of dsDNA in 50 µL assay volume).

The Minicell Adaptor Kit comes equipped with 500 borosilicate, flat-bottomed vials that are easily loaded in and out of the adaptor. The disposable vials eliminate potential cross-contamination and time-consuming cleanup required with non-disposable cells. Vials accommodate a sample volume range of 50-250 µL.


Results from the Minicell Adaptor used to quantitate DNA with Molecular Probes PicoGreen^® Assay.

Minimum assay volume	10 x 10 mm cuvette 2 mL	Minicell 50 µL
Minimum Detection Limit	500 pg/mL	1 ng/mL
Minimum amount of DNA required	1 ng	50 pg
Cost of the reagent per sample	$1.20	$0.03

Benefit of using Minicell with the PicoGreen Assay.

Straightforward easy to use Fluorometer
The TBS-380 Mini Fluorometer is designed for fast accurate fluorescence measurements. Unlike larger, more complex fluorometers, it is so easy to use that you can have results within minutes without training or previous experience. The single point calibration saves time, and the dual-channel design allows you to switch between two assays with a single press of a button.

TBS-380 PicoGreen and RiboGreen Concentration Graphs

Low Cost Fluorometer
Small in size but big in utility, the TBS-380
Mini-Fluorometer gives you the performance you expect from larger and more expensive instruments. Its inexpensive price meets your need for accurate fluorescence measurements within a limited budget. Although quite economical, the TBS-380 Mini-Fluorometer provides superior sensitivity and reliability.

FLUORESCENCE APPLICATION METHODS

DNA Quantitation using PicoGreen^{® and the
TBS-380 Mini Fluorometer}
(S-0070)
RNA Quantitation using RiboGreen^® ^{the TBS-380 Mini Fluorometer}(S-0071)
DNA Quantitation using Hoechst 33258 Dye ^{the
TBS-380 Mini Fluorometer} (S-0072)
Protein Quantitation using NanoOrange™ ^{the
TBS-380 Mini Fluorometer}
(S-0073)

TBS-380 MINI FLUOROMETER SPECIFICATIONS

Detector:	Silicon photodiode.
Sensitivity	1 ng/mL DNA using PicoGreen^®, 1 ng/mL RNA using RiboGreen^®, and 10 ng/mL DNA using Hoechst 33258 in a standard 10 x 10 mm cuvette and 50-200 µL Minicell. 100 ng/mL protein using NanoOrange™ in a standard 10 x 10 mm cuvette.
Light Source	UV LED 365—395 nm, Blue LED 465—485 nm
Sample Compartment	Accepts 10 x 10 x 45 mm square plastic cuvettes, optional 50—200 µL
Readout	Direct concentration.
Calibration	Single-point calibration.
Discrete Sample Averaging	Sample readings are averaged to improve accuracy.
Dynamic Range	~ 10e3
Data Output	ASCII format through a 9-pin RS-232 serial cable at 9600 baud for PC and MAC
Operating Temperature	59—98 °F; 15—35 °C.
Weight	0.7 kg (1.8 lb).
Dimensions:	14 cm W x 18.42 cm D x 6.9 cm H (5.5" W x 7.25" D x 2.7" H).
Power:	9 Volt external power supply (UL approved), 100—240 V AC, 50/60 Hz, max. 5 Watts.
Warranty	One-year warranty.
Approvals	CE.
Printer	Serial Printer (optional).

Applicable Fluorescent Reporters

	Ordering Information
Model Number	Description	Price US$
3800-003	UV & Blue Channel Internal data logging Solid Standard Instructions	$2650.00
3800-928	Minicell adaptor kit for 50-250 ul samples	$550.00
7000-950	Minicell Borosilicate glass cuvettes - 400	$175.00
7000-957	10 x 10 mm polystyrene cuvette 3.5 ml - 100 per pack	$95.00
7000-959	10 x 10 mm methacrylate cuvette 3.5 ml - 100 per pack	$80.00
7000-919	Serial Printer - thermal	$525.00
7000-918	Thermal Printer paper 1 roll	$15.00

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Tel: 781 740 8778 Fax: 781 740 8779

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