Denaturing Gradient Gel Electrophoresis

gel electrophoresisThe TV-400-DGGE denaturing gradient gel electrophoresis system is a cost-effective solution for researchers studying mutations and DNA polymorphisms critical in disease aetiology and genetic diversity. Designed primarily for parallel denaturing gradien gel elctrophoresis, where electrphoresis and the denaturing gradient run in the same direction, the TV400-DGGE has a maximun 96-sample throughput compatible with standard microplates and thermal cycler blocks, A 100ml gradient mizer is also included to pour gradient gels using our newly designed gel-running module and cam-caster, while a 400W heater, regulated by an external temperature control unit connected to a heat sensor within the gel tank, allows the gel temperature to be set to the predetermined melting temperature(Tm) of the PCR amplified DNA polymorphism or mutation of interest.
Heater elements
Temperature Controller
Benefits Include:
  • Maximum 96 Sample Throughput-
    using two 48-sample combs- allows samples to be transferred quickly and easily to each gel from standard 96-well microplates or thermal cycler blocks
  • Large Format-
    20.5x 20cm glass plates allow gradient gels to be poured containing wider ranges of denaturant concentration, maximizing sensitivity and resolution
  • 100ml gradient mixer-
    used with the new-style TV400-GRM and casting base to make two 2mm parallel denaturing gradient gels
  • can also be adapted for constant denaturing gel electrophoresis (CDGE)-
    if the denaturent concentration required for partial melting of the DNA target sequence is already known
  • Parallel DGGE Overview:
    Parallel DGGE is based on the application of a denaturant concentration gradient - increasing in the direction of electrophoresis within a polyacrylamide gel - and uniform temperature to induce the partial unwinding or melting of double-stranded DNA by distinct domains in a sequence-specific manner. Melting breaks the hydrogen bonds holding together each base pair within a DNA sequence: with domains rich in GC base pairs melting at higher temperatures than those with high A-T base-pair content.

    Incorporating sequence high in G-C content into the DNA target sequence by PCR® amplification (GC-clamping) prevents the DNA target with the lowest melting domain from melting completely at its predetermined Tm, which is maintained by the application of a constant temperature across the gel and within the buffer. Because both gel temperature and increasing denaturant concentration act to melt DNA in a sequenceand domain-specific manner, any point mutations amplified within a PCR® product will affect its Tm so that its subsequent mobility within a polyacrylamide gel differs from its wild type counterpart (see schematic diagram).

    Consequently, the sensitivity of this technique in distinguishing individual DNA sequences, which might differ only by a single point mutation, has proven highly attractive to scientists screening for unknown mutations.

    Complete System Includes:

    • Twin-plate maxi-gel unit with GRM, heated gel tank with PT100 temperature sensor and thermometer, lid,
    • 2 x (20.5 x 20cm; W x H) plain glass plates
    • 2 x (20.5 x 20cm) notched glass plates, 4 x 1mm spacers,
    • 2 x spacer aligners and 2 x 1mm thick 24-sample combs,
    • casting base, 2 x silicone seals and 1 x dummy plate;
    • plus GM100 gradient mixer and heat sensor control unit
    TV400 specs

    Ordering Information Buy Online
    TV400-DGGE Complete DGGE system with accessory kit and Casting Base 110Volts $2450.00
      Recommended power supply EV245 (replaces EV243) $699.00
    Combs Click for combs  

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